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How to exclude high-pressure liquid chromatography pump bubb

Source:未知 Posted:2015-08-12 23:25 Browse:

Conducting liquid chromatography analysis experiments, the mobile phase before re-entering the instrument, you must use an ultrasonic cleaner for simple Bubble treatment, to prevent pump damage, interfere with the test results. If it is found during a test liquid chromatograph constant flow pump in more air bubbles to be timely exclusion, exclusion high pressure liquid chromatography pump in the bubble as follows.
(1) First, check the mobile phase reservoir bottle of solvent filters are not clogged. Specifically: the input solvent plastic tee on the connecting tube removed, the mobile phase reservoir bottle in the high, use of pressure to the solution flow. If the solvent flow is very smooth, indicating good solvent filters. If it is flowing drop by drop, then the head of line blocking filter is required ultrasonic cleaning, restoration.
(2) high pressure instrument plug flow output mouth shut (the column even in the pipeline), can be avoided when the suction air is pumped into the pipeline. The instrument with the catheter syringes and plastic connecting pipe into the vent valve, open air valve knob counter-clockwise, pumping fluid 5-10mL, the pump flow rate is set at one milliliter per minute flow rate. Start the pump, observe the flow of the solution if there is a bubble plastic connecting tube. As no air bubbles from the filter head to illustrate the vent valve that has been bubble period of exclusion.
(3) the pump is turned off, the plug flow output from the high pressure port of the instrument to remove (the column removed from the pipeline) the output port vented. Close the vent valve knob clockwise, the pump flow rate is set at a flow rate of 3-5mL will bubble damper and pressure sensors inside the pump expelled effluent solution 10-15mL the pump off. Connection on the column, the flow rate was set at one milliliter per minute, starting the pump, observe the pressure and pressure pulsation.
(4) water and isopropyl alcohol (methanol) alternately flushing pump cavity, the bubble adsorption on the inner wall of eviction.
(5) The application of rapid depressurization expelled bubble: plug the flow with a small pump with outlet plug, the flow rate was set at 1 ml / min, the pre-column pressure rose to about 20Mpa, the drain valve fast open, repeat 2 or 3 can be.