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LC constant flow pump troubleshooting process

Source:未知 Posted:2015-08-12 23:08 Browse:

Constant high pressure pump is a reciprocating dual piston pumps in parallel controlled by a microcomputer, easy to operate, stable flow rate, pressure pulsation, low failure rate, it can play well transfusion unit HPLC system role. Performing chromatography analysis experiment, the mobile phase solution residual impurities damage the pump. The following brief analysis of liquid chromatography current pump troubleshooting methods and processes.
1. The pump is replaced by the mobile phase and purified water, will be filled with purified water reservoir bottle in the high pressure to form a bit. Unscrew to remove the mobile phase inlet pipe screws, observe the input from the mobile phase flowing out of the nozzle flow rate of purified water; if purified water flow is very smooth, indicating that the filter head is no problem. Purified water flow rate If the flow is very slow or drop by drop, drop down, indicating that the filter head needs to be cleaned (ultrasonic cleaning machine processing).
2. Place the mobile phase infusion tube Replace input tees. Plug flow with high pressure outlet blocked (or connect the column) to open the vent valve, with a 30mL syringe bubbles from the lines drawn (volume extracted about 5 ~ 6mL), close the vent valve, remove the pressure fluid plug (Remove the column) flow outlet on the connection one (1/16 "OD, 0.01" 400mm blue) PEEK pipe, the flow rate was set to open in quick rinse pump, the pump is driven from the inside of the bubble (20mL) off the pump. With a high pressure liquid stream outlet plug plugged tighten the flow rate was set at 0.5mL / min, the pressure was raised to start the pump 20Mpa, shut down the pump. Display observe the pump pressure, within 10 minutes of not less than 0.3Mpa (observe the connection pipe is leaking phenomenon), it can be considered a one-way valve is not leaking output. If the pressure is lower than the above value, the output may be manual purge check valve.
3. Open the pump housing, in the high-pressure flow output port connection on the column. The flow rate was set at 1.0mL / min, start the pump, observe the left and right head of high and low pressure changes. If the left and right below the head pressure head pressure, stop the pump. Removing the lower inlet check valve left pump head, according to the instructions of the cleaning head left inlet check valve and reinstall. Right pump head input process and said the same as the one-way valve. Retest the left and right pump pressure changes in the level, generally 0.2Mpa.
4. processed through the left and right pump inlet check valves. After re-test pressure change the left and right of the pump head is still more than 0.2Mpa. Position switching left and right pump inlet check valve (check valve left of the input pump head, change the pump head to the right; the right to enter the one-way valve head and change the pump head to the left.) Test As a result there are two cases.
5. After the exchange, the former head of the left and right of the pump head pressure is lower than the pressure becomes lower than the left and right head pressure head pressure. This shows that the head of the inlet check valve left no clean or is damaged and need replacing.
6. After an exchange, the former head of the left and right pressure is lower than the pressure of the pump head, or head pressure is lower than the original left and right head pressure. This shows that the high pressure seal left pump head needs to be replaced. (Elution volume can be measured to determine if high pressure seal leakage)
7. If there is a pressure value as large as the left and right pump changes, it is the problem of the damper.
8. If the output of the one-way valve in the cleaning, I found particularly difficult when the drainage Hou is the output of the one-way valve 2um filters clog, require ultrasonic cleaning. If you need to replace ineffective. (If there is a one-way valve output filters completely blocked, the other output filters check valve is not completely blocked. The two output filters to replace all one-way valve to avoid a wrong impression to the customer at the time of the instrument. ʱ??
9. damper problem: When the normal outflow solution from the discharge port; drain valve is closed, the pump outlet No solution flow, no pressure display. After the pump is running at 1ml / min flow rate of 2 to 3 minutes, open the drain valve, there is a solution spray. Description damper endometrial adhesions or blocked, in need of repair damper.