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GC and LC What is the difference?

Source:未知 Posted:2015-08-12 23:36 Browse:

Gas chromatography and liquid chromatography, what's the difference? Usually GC is to analyze a certain volatile substances, liquid chromatography to analyze the organic compounds in general, but is not well adapted too volatile, liquid chromatography apparatus for high boiling point, polar material advantage. If you want to go into detail, it can be distinguished from separation principles, applications, equipment construction of these three areas. Wuhan Hanson Century Technology Co., Ltd. today just as we talk about the difference between the gas chromatograph and liquid chromatography.
First, gas chromatography and liquid chromatography separation principle of distinction:
    1. Gas: Gas chromatography is a physical separation method. Use each component of the test substance in two different phases partition coefficient (solubility) of minor differences, when the two-phase relative movement of these substances are allocated in two phases repeated many times, so that the original only minor differences in the nature have a great effect, leaving the different components be separated.
    2. Liquid: HPLC is based on classic chromatography, gas chromatography reference to the theoretical, technically, the mobile phase changed to high pressure transmission (up conveying pressure up 4.9'107Pa); column is In a special way filled with small particle size of the filler is made so that the column efficiency is much higher than classical liquid chromatography (Ermita plate per up to tens or hundreds of thousands); and even after the column has a high sensitivity detection , which can be continuously detected for effluent.
Second, gas chromatography and liquid chromatography instrumentation applications range difference:
    1. Gas: gas chromatography having a separation capability, high sensitivity, rapid analysis, easy operation, but technical limitations, high boiling point, poor thermal stability of the substance or substances are difficult to use for analysis by gas chromatography ʱ?? Material part generally below 500 ℃ less volatile or heat labile can be derivatized or lysis method.
    2. Liquid: high performance liquid chromatography, only requires the sample solution can be made, without the need for gasification, and therefore not subject to the limitations of volatile samples. For high boiling point, poor thermal stability, relatively high molecular weight (greater than 400 or more) of organic matter (some substances accounted for almost 75% to 80% of the total organic matter) can be applied to high-performance liquid chromatography separation, analysis on principle. According to the statistics,
Third, gas chromatography and liquid chromatography instrument configuration differences:
    1. Gas: the carrier gas supply, injection parts, column, column oven, the detector and data processing system. Injection section, column and detector temperatures were in control.
    2. Liquid: high performance liquid chromatography mainly injection systems, infusion systems, separation systems, detection systems and data processing system.